Optimization of Medium Volume and Temperature for Improved α-amylase Production by Saccharomyces cerevisiae GCB-20
نویسندگان
چکیده
In the present study, selection of an appropriate volume and temperature for α-amylase production by Saccharomyces cerevisiae GCB-20 has been reported. The fermentation medium at the level of 15-40 ml was investigated in 250 ml conical flasks for enzyme production. Maximum α-amylase production (210 IU/ml/min) by S. cerevisiae GCB-20 was obtained in 25 ml of fermentation medium at 35°C. The kinetic analysis indicated that the volumetric production of biomass and α-amylase was significantly improved at the optimized fermentation conditions.
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